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propidium iodide pi dye exclusion assay  (Thermo Fisher)


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    Thermo Fisher propidium iodide pi dye exclusion assay
    Propidium Iodide Pi Dye Exclusion Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/propidium+iodide+pi+dye+exclusion+assay/pm17210217-72-14-11
    Average 86 stars, based on 1 article reviews
    propidium iodide pi dye exclusion assay - by Bioz Stars, 2026-09
    86/100 stars

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    Exclusion Assay:

    Article Title: Cell death and cytotoxic effects in YAC-1 lymphoma cells following exposure to various forms of mercury.
    Article Snippet: The effects of 1 min–4 h exposures to four Hg compounds (mercuric chloride [HgCl2], methyl mercuric chloride [CH3HgCl], pchloromercuribenzoate [p-CMB] and thimerosal [TMS; ethylmercurithiosalicylate]) on cell death, microtubules, actin, CD3 receptor expression, protein tyrosine phosphorylation (PTyr-P) and intracellular calcium ([Ca]i) levels were investigated in YAC-1 lympoma cells using flow cytometry.. YOPRO-1 (YP) and propidium iodide (PI) dye uptake indicated all forms of Hg tested were toxic at concentrations ranging from 25.8–48.4 M, with two distinct patterns of effects.. Early apoptosis was prolonged for CH3HgCland TMS-treated cells, with more than 50% remaining YP+/PI− after 4 h. Both CH3HgCl and TMS induced complete loss of -tubulin fluorescence, indicative of microtubule depolymerization and inhibition of tubulin synthesis and/or -tubulin degradation, while F-actin fluorescence diminished to a lesser degree and only after loss -tubulin.



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    propidium iodide (pi) dye exclusion  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc propidium iodide (pi) dye exclusion
    Plasma membrane profiling (PMP) by mass spectroscopy identifies ABCG2. (A) Table of top hits identified using PMP to compare HMB-PP treated and untreated HeLa-M cells. Several independent peptides of ATP-binding cassette (ABC) transporter ABCG2 were detected, with modest increase (log 2 2.69) by treatment. Other potential candidates by these criteria (C3orf52 and FAM129B) are listed and were less significant. (B) Dysregulation of a subset of ABC transporters, including ABCG2, in HeLa cells detected by RT-PCR. Template cDNA was prepared from total RNA from HeLa-L and HeLa-M cells left untreated or treated with γδ T cell supernatant for 24 h. RT-PCR products were analyzed by gel electrophoresis. (C) Variation in ABCG2 protein expression in HeLa cells by western blot. Top panel: HeLa-L and HeLa-M cells were left untreated or treated with MG132 (5 µM for 10 h) or HMB-PP (10 nM for 10 h). Cell lysates were prepared and analyzed using antibodies directed to ABCG2, NRF2, and calnexin (CNX), which acts as a loading control. Bottom panel: western blot analysis of shRNA ABCG2 cells confirms reduction in ABCG2 protein expression. (D) HeLa-M cells show ABCG2-dependent resistance to cytotoxic drug doxorubicin (DOX). Wild-type HeLa-M, HeLa-L, and shRNA ABCG2 HeLa-M cells were incubated (24 h) with DOX at the indicated concentrations. Cells were stained using <t>propidium</t> iodide (PI) (2.5 µg/ml for 5 min) as a marker of plasma membrane integrity. After washing, cells were analyzed by cytometry. Chart shows mean fluorescence intensity (MFI) of PI staining. (E) No effect on T cell activation using shRNA ABCG2 HeLa-M cells. The effect on the efficiency of Vγ9/Vδ2 T cell activation for a series of HeLa-M gene knockdown lines, including shRNA BTN3A1, ABCG2, RhoB, PLEC1, and TRIM21. Cells were pretreated with HMB-PP (10 nM for 6 h) before Vγ9/Vδ2 T cells from a single donor were applied. Coculture was allowed to proceed overnight when culture supernatants were analyzed by ELISA. Chart shows percent (%) change in detected IFN-γ relative to wild-type HeLa-M cells of duplicate determinations for each cell line.
    Propidium Iodide (Pi) Dye Exclusion, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/propidium+iodide+pi+dye+exclusion+assay/propidium+iodide+pi/pmc05893821-50-4-15
    Average 90 stars, based on 1 article reviews
    propidium iodide (pi) dye exclusion - by Bioz Stars, 2026-09
    90/100 stars
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    propidium iodide pi dye exclusion assay  (Thermo Fisher)
    86
    Thermo Fisher propidium iodide pi dye exclusion assay
    Plasma membrane profiling (PMP) by mass spectroscopy identifies ABCG2. (A) Table of top hits identified using PMP to compare HMB-PP treated and untreated HeLa-M cells. Several independent peptides of ATP-binding cassette (ABC) transporter ABCG2 were detected, with modest increase (log 2 2.69) by treatment. Other potential candidates by these criteria (C3orf52 and FAM129B) are listed and were less significant. (B) Dysregulation of a subset of ABC transporters, including ABCG2, in HeLa cells detected by RT-PCR. Template cDNA was prepared from total RNA from HeLa-L and HeLa-M cells left untreated or treated with γδ T cell supernatant for 24 h. RT-PCR products were analyzed by gel electrophoresis. (C) Variation in ABCG2 protein expression in HeLa cells by western blot. Top panel: HeLa-L and HeLa-M cells were left untreated or treated with MG132 (5 µM for 10 h) or HMB-PP (10 nM for 10 h). Cell lysates were prepared and analyzed using antibodies directed to ABCG2, NRF2, and calnexin (CNX), which acts as a loading control. Bottom panel: western blot analysis of shRNA ABCG2 cells confirms reduction in ABCG2 protein expression. (D) HeLa-M cells show ABCG2-dependent resistance to cytotoxic drug doxorubicin (DOX). Wild-type HeLa-M, HeLa-L, and shRNA ABCG2 HeLa-M cells were incubated (24 h) with DOX at the indicated concentrations. Cells were stained using <t>propidium</t> iodide (PI) (2.5 µg/ml for 5 min) as a marker of plasma membrane integrity. After washing, cells were analyzed by cytometry. Chart shows mean fluorescence intensity (MFI) of PI staining. (E) No effect on T cell activation using shRNA ABCG2 HeLa-M cells. The effect on the efficiency of Vγ9/Vδ2 T cell activation for a series of HeLa-M gene knockdown lines, including shRNA BTN3A1, ABCG2, RhoB, PLEC1, and TRIM21. Cells were pretreated with HMB-PP (10 nM for 6 h) before Vγ9/Vδ2 T cells from a single donor were applied. Coculture was allowed to proceed overnight when culture supernatants were analyzed by ELISA. Chart shows percent (%) change in detected IFN-γ relative to wild-type HeLa-M cells of duplicate determinations for each cell line.
    Propidium Iodide Pi Dye Exclusion Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/propidium+iodide+pi+dye+exclusion+assay/pm17210217-72-14-11
    Average 86 stars, based on 1 article reviews
    propidium iodide pi dye exclusion assay - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

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    Plasma membrane profiling (PMP) by mass spectroscopy identifies ABCG2. (A) Table of top hits identified using PMP to compare HMB-PP treated and untreated HeLa-M cells. Several independent peptides of ATP-binding cassette (ABC) transporter ABCG2 were detected, with modest increase (log 2 2.69) by treatment. Other potential candidates by these criteria (C3orf52 and FAM129B) are listed and were less significant. (B) Dysregulation of a subset of ABC transporters, including ABCG2, in HeLa cells detected by RT-PCR. Template cDNA was prepared from total RNA from HeLa-L and HeLa-M cells left untreated or treated with γδ T cell supernatant for 24 h. RT-PCR products were analyzed by gel electrophoresis. (C) Variation in ABCG2 protein expression in HeLa cells by western blot. Top panel: HeLa-L and HeLa-M cells were left untreated or treated with MG132 (5 µM for 10 h) or HMB-PP (10 nM for 10 h). Cell lysates were prepared and analyzed using antibodies directed to ABCG2, NRF2, and calnexin (CNX), which acts as a loading control. Bottom panel: western blot analysis of shRNA ABCG2 cells confirms reduction in ABCG2 protein expression. (D) HeLa-M cells show ABCG2-dependent resistance to cytotoxic drug doxorubicin (DOX). Wild-type HeLa-M, HeLa-L, and shRNA ABCG2 HeLa-M cells were incubated (24 h) with DOX at the indicated concentrations. Cells were stained using propidium iodide (PI) (2.5 µg/ml for 5 min) as a marker of plasma membrane integrity. After washing, cells were analyzed by cytometry. Chart shows mean fluorescence intensity (MFI) of PI staining. (E) No effect on T cell activation using shRNA ABCG2 HeLa-M cells. The effect on the efficiency of Vγ9/Vδ2 T cell activation for a series of HeLa-M gene knockdown lines, including shRNA BTN3A1, ABCG2, RhoB, PLEC1, and TRIM21. Cells were pretreated with HMB-PP (10 nM for 6 h) before Vγ9/Vδ2 T cells from a single donor were applied. Coculture was allowed to proceed overnight when culture supernatants were analyzed by ELISA. Chart shows percent (%) change in detected IFN-γ relative to wild-type HeLa-M cells of duplicate determinations for each cell line.

    Journal: Frontiers in Immunology

    Article Title: Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters

    doi: 10.3389/fimmu.2018.00662

    Figure Lengend Snippet: Plasma membrane profiling (PMP) by mass spectroscopy identifies ABCG2. (A) Table of top hits identified using PMP to compare HMB-PP treated and untreated HeLa-M cells. Several independent peptides of ATP-binding cassette (ABC) transporter ABCG2 were detected, with modest increase (log 2 2.69) by treatment. Other potential candidates by these criteria (C3orf52 and FAM129B) are listed and were less significant. (B) Dysregulation of a subset of ABC transporters, including ABCG2, in HeLa cells detected by RT-PCR. Template cDNA was prepared from total RNA from HeLa-L and HeLa-M cells left untreated or treated with γδ T cell supernatant for 24 h. RT-PCR products were analyzed by gel electrophoresis. (C) Variation in ABCG2 protein expression in HeLa cells by western blot. Top panel: HeLa-L and HeLa-M cells were left untreated or treated with MG132 (5 µM for 10 h) or HMB-PP (10 nM for 10 h). Cell lysates were prepared and analyzed using antibodies directed to ABCG2, NRF2, and calnexin (CNX), which acts as a loading control. Bottom panel: western blot analysis of shRNA ABCG2 cells confirms reduction in ABCG2 protein expression. (D) HeLa-M cells show ABCG2-dependent resistance to cytotoxic drug doxorubicin (DOX). Wild-type HeLa-M, HeLa-L, and shRNA ABCG2 HeLa-M cells were incubated (24 h) with DOX at the indicated concentrations. Cells were stained using propidium iodide (PI) (2.5 µg/ml for 5 min) as a marker of plasma membrane integrity. After washing, cells were analyzed by cytometry. Chart shows mean fluorescence intensity (MFI) of PI staining. (E) No effect on T cell activation using shRNA ABCG2 HeLa-M cells. The effect on the efficiency of Vγ9/Vδ2 T cell activation for a series of HeLa-M gene knockdown lines, including shRNA BTN3A1, ABCG2, RhoB, PLEC1, and TRIM21. Cells were pretreated with HMB-PP (10 nM for 6 h) before Vγ9/Vδ2 T cells from a single donor were applied. Coculture was allowed to proceed overnight when culture supernatants were analyzed by ELISA. Chart shows percent (%) change in detected IFN-γ relative to wild-type HeLa-M cells of duplicate determinations for each cell line.

    Article Snippet: Killing assay was by propidium iodide (PI) dye exclusion in cells treated with doxorubicin DOX (Cell Signaling).

    Techniques: Clinical Proteomics, Membrane, Mass Spectrometry, Binding Assay, Reverse Transcription Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Expressing, Western Blot, Control, shRNA, Incubation, Staining, Marker, Cytometry, Fluorescence, Activation Assay, Knockdown, Enzyme-linked Immunosorbent Assay